Immunoprecipitation

Immunoprecipitation is used to precipitate out a protein of interest when this particular protein is in a solution with a whole bunch of other proteins or for example in a cell lysate. Antibodies are used to bind to the particular protein of interest and then this complex is precipitated out. Chromatin Immunoprecipitation (ChIP) uses the same concept except for the protein of interest is attached to chromatin. This technique is useful for determining how much of a particular protein binds to DNA in order to turn on a specific gene of interest.

If you want the complete protocol, here ya go…http://www.abcam.com/ps/pdf/protocols/Immunoprecipitation%20protocol%20(IP).pdf