Chp 6 HW

6.7.
Replication bubble explained on page 199 of text
5'-b-to-a-3'=leading
5'-e-to-f-3'=lagging
5'-g-to-h-3'=leading
5'-d-to-c-3'=lagging

15. This shows that the bacterial DNA replication is not semiconservative like all organisms on earth. it seems as though the bacter made a replicative DNA molecule exactly like the original heavy DNA w/out using it as a template (it just knew how to make exact DNA replications from the media containing N14)

17.
1. DNA Ligase=e
2. DNA polymerase=d
3. DNA helicase=a
4. RNA polymerase=c
5. Restriction enzyme=b

18. Because gene conversion is a result of mismatch repair, if G's decrease by 5%, one would assume A's would increase by 5%
EX: CGGAT….this is a mismatched pair so it is corrected to CGAAT
……..GCTTA…………………………………………………………………………..GCTTA
Since G is only likely to be mismatched with T's (b/c its a pyrimidine like C) it will be replaced by A. Thats why if one decreases, the other increases by the same amount.

19. She left out DNA ligase b.c this is supposed to join segmented DNA, mainly in the case where a RNA primer is encountered on the lagging strand.

20. DNA replication would go about normally at 37 C w/all DNA connected. At 42C, ligase wouldnt work and DNA would be segmented, which would lead to incomplete replication. The higher heat wouldn't allow it to be able to grow

29. a. True-according to Chargaff's rule, A=T, C=G, so A+C=G+T
b. True-same as part a
c. True-all purines should = all pyrimidies like in b.

Chargaff's Rules (page 43 in text)
[A]=[T]
[G]=[C]
[A]+[G]=[T]+[C]

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